The viral hit by South Korean star Psy topped the charts worldwide over the summer and landed him a deal with Justin Bieber's label Schoolboy Records.
'Gangnam Style' topped our reader-voted list with 14.6% of the vote, while Gotye's 'Somebody That I Used to Know' came second with 12.8%.
fun.'s 'We Are Young' finished in third place with 12.1%, Carly Rae Jepsen's 'Call Me Maybe' is fourth with 9% and Florence + the Machine round off the Top 5 with 7.7% of the vote for 'Spectrum'.
Over 25,000 readers voted in Digital Spy's annual end of year poll, which included categories for music, film, television, showbiz, gaming, tech and soaps.
Psy's 'Gangnam Style' has become one of the biggest online hits across the world in 2012, and is expected to be the first video to reach a billion views on YouTube later today (December 21).
Watch the music video for Psy's 'Gangnam Style' below: