Recent details of the episode revealed a plot revolving around Kabletown's refusal to allow Liz Lemon (Tina Fey) to air her sketch comedy series TGS live due to the expensive cost.
TVLine reports that Glover will be playing a younger version of Tracy Morgan's character on the show.
Glover was previously a writer on The Daily Show and 30 Rock.
He currently stars on the NBC comedy Community, and recently made his musical debut under the stage name Childish Gambino.
Community, which also stars Joel McHale, Allison Brie, and Gillian Jacobs, is though to be in contention for a fourth season despite weak ratings earlier in the year.
The live episode of 30 Rock is scheduled to air on April 26 on NBC.
Watch a clip from a recent episode of 30 Rock below: